fexA, a novel Staphylococcus lentus gene encoding resistance to florfenicol and chloramphenicol.
نویسندگان
چکیده
The Staphylococcus lentus plasmid pSCFS2 carries a novel florfenicol-chloramphenicol resistance gene, designated fexA, encoding a protein of 475 amino acids with 14 transmembrane domains. The FexA protein differs from all previously known proteins involved in the efflux of chloramphenicol and florfenicol. Induction of fexA expression by chloramphenicol and florfenicol occurs via translational attenuation.
منابع مشابه
Florfenicol-chloramphenicol exporter gene fexA is part of the novel transposon Tn558.
The florfenicol-chloramphenicol exporter gene fexA is part of the novel transposon Tn558 from Staphylococcus lentus. Similarities between Tn558 and Tn554 from Staphylococcus aureus included the arrangement of the transposase genes tnpA to -C and an att554-like target sequence. Circular forms of Tn558 were detected and suggest the functional activity of this transposon.
متن کاملDistribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates.
A total of 302 chloramphenicol-resistant Staphylococcus isolates were screened for the presence of the florfenicol/chloramphenicol resistance genes fexA and cfr and their localization on mobile genetic elements. Of the 114 isolates from humans, only a single Staphylococcus aureus isolate showed an elevated MIC to florfenicol, but did not carry either of the known resistance genes, cfr or fexA. ...
متن کاملIdentification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri.
The 16.5-kbp plasmid pSCFS1 from Staphylococcus sciuri mediated combined resistance to chloramphenicol and florfenicol. The gene responsible for this resistance property, cfr, was cloned and sequenced. The amino acid sequence of the Cfr protein revealed no homology to known acetyltransferases or efflux proteins involved in chloramphenicol and/or florfenicol resistance or to other proteins whose...
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During a study of florfenicol-resistant porcine staphylococci from Denmark, the genes cfr and fexA were detected in the chromosomal DNA or on plasmids of Staphylococcus hyicus, Staphylococcus warneri, and Staphylococcus simulans. A novel variant of the phenicol resistance transposon Tn558 was detected on the ca. 43-kb plasmid pSCFS6 in S. warneri and S. simulans isolates. Sequence analysis of a...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 48 2 شماره
صفحات -
تاریخ انتشار 2004